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Effects of NMDA receptor modulators on cell death in models of excitotoxicity in vitro.
Strnadová, Lenka ; Smejkalová, Tereza (advisor) ; Skřenková, Kristýna (referee)
NMDA receptors are ionotropic glutamate receptors (iGluR) activated by the amino acid glutamate and mediating signal transmission in the central nervous system. Their proper activity is essential for synaptogenesis, neuronal plasticity and synaptic transmission. However, excessive activation of NMDAR causes strong influx of calcium ions into neurons which triggers several destructive effects, eventually ending with cell death. This so-called excitotoxicity is present not only in many neurodegenerative diseases, such as Alzheimer's disease or Parkinson's disease, but also in acute pathophysiological conditions, such as stroke or traumatic brain injury. General NMDAR inhibitors that could potentially prevent neuronal excitotoxicity have shown severe negative side effects in models in vivo. On the other hand, selective inhibitors of NMDA receptors with the ability to block the unwanted excessive activity while preserving NMDAR physiological function have shown to be therapeutically useful. This work is going to briefly summarize the knowledge of structure, activation and localization of NMDA receptors, then it is going to describe their rule in mediating neuronal toxicity and a few methods we can use to study excitotoxicity in vitro. Finally, this work will compare the effects of several known NMDAR...
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Open Government Data
Kučera, Jan ; Jandoš, Jaroslav (advisor) ; Richta, Karel (referee) ; Šaloun, Petr (referee)
This Ph.D. thesis deals with Open Government Data and the methodology for publication of this kind of data. Public sector bodies hold a significant amount of data that can be reused in innovative way leading to development of new products and services. According to the Open Knowledge Foundation "Open data is data that can be freely used, re-used and redistributed by anyone - subject only, at most, to the requirement to attribute and sharealike." Publication and reuse of Open Government Data can lead to benefits such as increased economic growth. State, society as well as the public sector bodies themselves can benefit from Open Government Data. However the public sector bodies currently face a number of problems and issues when publishing Open Government Data, e.g. regular updates of the published datasets are not always ensured. Different public sector bodies apply different approaches to publication of Open Government Data. The main goal of this thesis is to design the Open Government Data Publication Methodology which should address current problems related to the publication of Open Government Data.
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